Struik, P. C. 2007. Above-ground and below-ground plant development. Potato biology and biotechnology : advances and perspectives. Vreugdenhil, D. and et al. Amsterdam, Elsevier: ۲۱۹-۲۳۶٫
Struik, P. C. and Wiersema, S. G. 2002. Seed potato technology. Wageningen Press. 383pp
Tovar, P., Dodds, J. H. and Chandre, D. E. R. 1987. Effect of medium composition on in vitro tuberization. potato Abs 13(6).
Tovar, P., Estrada, R., Schilde-Rentschler, L. and Dodds, J. H. 1985. Induction and use of in vitro potato tubers. CIP Circular 13(4): 1-5.
wang, P. J. and Hu, C. Y. 1982. in vitro mass tuberization and virus-free seed potato production in taiwan. Amer. Potato 60: 27-33.
Wang, P. J. and Hu, C. Y. 1985. Potato tissue culture and its application in agriculture. Potato physiology Li, P. H. Academic press, New York, U.S.A.: ۵۰۳-۵۷۷٫
Yeasmin, L., Ahmad, S., Rashid, M. H., Parveen, S. and Zeba, N. 2011. Effect of nitrogen and potassium on in vitro development of microtuber of potato (Solanum tuberosum L.). Expt. Biosci 1: 107-112.
Yiem, M. S., Park, Y., H., Kim, J. K., Kim, S. Y., Cho, H. M. and Hahan, B. H. 1990. Studies on seed potato (Solanum tubresum) multiplication by microtuberization and practical use. Field crop Abs 44(10).
Yousef, A. A. R., Suwwan, M. A., Al Musa, A. M. and Ahu-Qaoud, H. A. 1997. In vitro culture and microtuberization of ‘Spunta’ potato (Solanum tuberosum L.) Agricultural Science 24(2): 173-181.
Yu, W. C. P. J., Joyce, D. C. and Cameron-McCown, B. H. 2000. Sucrose utilization during potato microtuber growth on bioreactors. Plant Cell reports 19: 407-413.
Zimmermann, R. H., Bhardwaj, S. V. and Fordham, I. M. 1995. Use of starch-gelled medium for tissue culture of some fruit crops. Plant cell tiss. Org. Cult 43: 207-213.
Potato (Solanum tuberosum L.) is one of the most important nutrients crop after wheat, rice and maize therefore, is the world’s fourth main product. Potato crop due to vegetative propagation is susceptible to contamination by bacteria, fungi, viruses and viroid. Integrated pathogens transmitted to the next generation of vegetative growth. In recent years, potato seed tuber production system by in vitro culture are designed and operated. Tissue culture methods can rapidly multiply and provide massive amounts of healthy and free pathogens seed tubers in short time. The aim of this study was to identify the effect of growth hormone regulator on in vitro potato plantlets and microtuberization production in order to increase the number of microtuber. The effect of PGR treatments applied by 14 independent experiments on Savalan, Sante, Agria and Markies cultivars. The effects of 2iP, BAP, CCC, GA3, Picholoram, NAA and hormone-free medium were studied on plantlet growth and microtuberization. Next step the effect of desirable concentrations hormone and their interactions on seedling growth and microtuberization production were studied by using response surface method in MS medium. According to the results concentration of 1.19 mg.l-1 GA3 and 0.60 mg.l-1 NAA for Savalan cultivar, concentration of 1.20 mg.l-1 GA3 and 0.60 mg.l-1 NAA for Santé and Agria cultivars, concentration of 0.60 mg.l-1 NAA for Markies cultivar elevated production of plantlets. The results showed for microtuber production the best result were observed in, 1000 mg.l-1 of CCC and for Agria cultivar concentration of 999.95 mg.l-1 CCC. The results demonstrated maximum growth was achieved in Savalan cultvar. Sante cultivars produced maximum amount of microtuber. Mrakies cultivars produced The largest microtubers.
Keywords: Potato, Plantlets, Microtuber, Tissue culture and Hormones.
Islamic Azad University
Faculty of Agriculture
A Thesis Submitted in partial Fullfillment of the requirement For
The Degree of M.sc. in Agricultural Biotechnology
Study and comparison of in-vitro microtuberization in early- and late-maturity cultivars of potato (Solanum tuberosum L.)
Dr. Majid Masoumian
Dr. Hossien Afshari
Ehsan Enayat Manesh
- . Spooner ↑
- . FAO ↑
- . Bowen ↑
- . Tovar ↑
- . Siddiqui ↑
- . Struik ↑
- . In vitro ↑
- . Beukema ↑
- . Zagg ↑